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Original Research (Original Article) 


The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats

Başak Baykara, Burak Baykara, Mehmet Ateş, İlkay Aksu, Müge Kıray, Ayşegül Taş, Hikmet Gümüş, Caner Çetinkaya, Ali Rıza Şişman, Durgül Yılmaz, Mehmet Nuri Arda, Nazan Uysal.

Abstract
Objective: The aim of this study is to investigate the relationship between anxiety due to traumatic brain injury (TBI) and prefrontal cortex serotonin (5-HT) and 5-HT2A receptor in immature rats. Methods: Seven days old rats were subjected to traumatic brain injury model. They were divided into five groups. 1-Sham; 2-TBI group; 3-TBI followed by 14 days of administration of essitalopram (selective serotonin reuptake inhibitors; SSRI) (10 mg/kg) group (TBI+SSRI); 4-TBI and cyproheptadine (nonspecific serotonin receptor antagonist; A) (5 mg/kg) given by gastric gavage one hour prior to behavioral tests (TBI+A); 5-TBI followed by 14 days of essitalopram (SSRI) and cyproheptadine (A) given 1 hour prior to behavioral tests (TBI+SSRI+A). Elevated T-maze test and open field test applied to all groups and then blood corticosterone, prefrontal cortex tissue 5-HT and 5-HT2A receptor quantities measured. Prefrontal cortex neuron density histologically evaluated. Results: In the TBI group, the time spent in the peripheral cells, the time spent in the elevated T-maze closed arms, and serum corticosterone levels found to increase as a result of anxiety. Neuronal density decreased in prefrontal cortex. SSRI treatment reduced the time spent on the closed arms in the elevated T-maze test. SSRI decreased serum corticosterone levels and increased neuronal density. Tissue serotonin levels decreased in all groups exposure to TBI compared to sham group. 5-HT2A receptor levels were higher in the TBI and A group. Conclusion: SSRIs showed anxiolytic effect for anxiety, secondary to TBI in immature rats.

Key words: immature rats, traumatic brain injury, prefrontal cortex, serotonin, serotonin 2A receptor, SSRI



İmmatür sıçanlarda travmatik beyin hasarı ile ortaya çıkan anksiyetede serotonin ve serotonin 2A reseptörünün rolü

Ozet
Amaç: Bu çalışmanın amacı immatür ratlarda, travmatik beyin hasarında ortaya çıkan anksiyetenin, prefrontal kortekste serotonin (5-HT) ve 5-HT2A reseptörü ile ilişkisini araştırmaktır. Yöntem: Yedi günlük yavru ratlara travma-tik beyin hasarı modeli uygulandı ve beş gruba ayrıldı: 1-Sham; 2-Travmatik beyin hasarı (TBH) grubu; 3-Travma uygulanıp ardından 14 gün, 10 mg/kg essitalopram (seçici serotonin gerialım inhibitörü; SSRI) verilen grup (TBH+SSRI grubu); 4-Travma uygulanıp davranış deneylerinden 1 saat önce gastrik gavaj ile siproheptadin (non-spesifik serotonin reseptör antagonisti, A) 5 mg/kg verilen grup (TBH+A grubu); 5-Travma uygulanıp ardından 14 gün essitalopram ve davranış deneylerinden 1 saat önce gastrik gavaj ile siproheptadin verilen grup (TBH+SSRI+A grubu). Tüm gruplara yükseltilmiş +labirent testi ve açık alan testi uygulanmış, sonrasında kan kortikosteron, pre-frontal korteks doku serotonin ve 5-HT2A reseptör miktarları ölçülmüştür. Prefrontal korteks nöron yoğunluğu değer-lendirilmiştir. Bulgular: TBH uygulanan grupta, açık alan testinde çevre hücrelerde geçirilen zaman ve yükseltilmiş +labirentin kapalı kollarında geçirilen zaman ve serum kortikosteron düzeyleri anksiyete ile doğru orantılı olarak artmış bulundu. Prefrontal kortekste nöron yoğunluğunda ise azalma gözlendi. SSRI tedavisi yükseltilmiş +labirent testinde kapalı kollarda geçirilen süreyi azalttı. Serum kortikosteron düzeylerini düşürdü ve nöron yoğunluğunu artır-dı. Doku serotonin düzeyleri, TBH uygulanan tüm gruplarda, sham grubuna göre azalmış olarak saptandı. 5-HT2A reseptör düzeyleri, TBH ve siproheptadin kullanan grupta yüksek bulundu. Sonuç: İmmatür ratlarda TBH’ye ikincil ortaya çıkan anksiyetede SSRI’lar anksiyolitik etki göstermiştir.

Anahtar Kelimeler: İmmatür rat, travmatik beyin hasarı, prefrontal korteks, serotonin, serotonin 2A reseptörü, SSRI


 
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How to Cite this Article
Pubmed Style

Baykara B, Baykara B, Ates M, Aksu I, Kiray M, Tas A, Gumus H, Cetinkaya C, Sisman AR, Yilmaz D, Arda MN, Uysal N. [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anadolu Psikiyatri Derg. 2019; 20(4): 368-376. Turkish. doi:10.5455/apd.302643454


Web Style

Baykara B, Baykara B, Ates M, Aksu I, Kiray M, Tas A, Gumus H, Cetinkaya C, Sisman AR, Yilmaz D, Arda MN, Uysal N. [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. http://www.anadolupsikiyatri.net/?mno=302643454 [Access: April 01, 2020]. Turkish. doi:10.5455/apd.302643454


AMA (American Medical Association) Style

Baykara B, Baykara B, Ates M, Aksu I, Kiray M, Tas A, Gumus H, Cetinkaya C, Sisman AR, Yilmaz D, Arda MN, Uysal N. [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anadolu Psikiyatri Derg. 2019; 20(4): 368-376. Turkish. doi:10.5455/apd.302643454



Vancouver/ICMJE Style

Baykara B, Baykara B, Ates M, Aksu I, Kiray M, Tas A, Gumus H, Cetinkaya C, Sisman AR, Yilmaz D, Arda MN, Uysal N. [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anadolu Psikiyatri Derg. (2019), [cited April 01, 2020]; 20(4): 368-376. Turkish. doi:10.5455/apd.302643454



Harvard Style

Baykara, B., Baykara, . B., Ates, . M., Aksu, . I., Kiray, . M., Tas, . A., Gumus, . H., Cetinkaya, . C., Sisman, . A. R., Yilmaz, . D., Arda, . M. N. & Uysal, . N. (2019) [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anadolu Psikiyatri Derg, 20 (4), 368-376. Turkish. doi:10.5455/apd.302643454



Turabian Style

Baykara, Basak, Burak Baykara, Mehmet Ates, Ilkay Aksu, Muge Kiray, Aysegul Tas, Hikmet Gumus, Caner Cetinkaya, Ali Riza Sisman, Durgul Yilmaz, Mehmet Nuri Arda, and Nazan Uysal. 2019. [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anatolian Journal of Psychiatry, 20 (4), 368-376. Turkish. doi:10.5455/apd.302643454



Chicago Style

Baykara, Basak, Burak Baykara, Mehmet Ates, Ilkay Aksu, Muge Kiray, Aysegul Tas, Hikmet Gumus, Caner Cetinkaya, Ali Riza Sisman, Durgul Yilmaz, Mehmet Nuri Arda, and Nazan Uysal. "[The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]." Anatolian Journal of Psychiatry 20 (2019), 368-376. Turkish. doi:10.5455/apd.302643454



MLA (The Modern Language Association) Style

Baykara, Basak, Burak Baykara, Mehmet Ates, Ilkay Aksu, Muge Kiray, Aysegul Tas, Hikmet Gumus, Caner Cetinkaya, Ali Riza Sisman, Durgul Yilmaz, Mehmet Nuri Arda, and Nazan Uysal. "[The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]." Anatolian Journal of Psychiatry 20.4 (2019), 368-376. Print.Turkish. doi:10.5455/apd.302643454



APA (American Psychological Association) Style

Baykara, B., Baykara, . B., Ates, . M., Aksu, . I., Kiray, . M., Tas, . A., Gumus, . H., Cetinkaya, . C., Sisman, . A. R., Yilmaz, . D., Arda, . M. N. & Uysal, . N. (2019) [The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats]. Anatolian Journal of Psychiatry, 20 (4), 368-376. Turkish. doi:10.5455/apd.302643454